| Synergistic Wound Healing Approaches: Integrated Findings from Three Parallel Studies Evaluating Photodynamic Therapy and Nanoparticle-Enhanced Laser Treatments in Murine Models |
| Paper ID : 1001-ICLA 11-FULL |
| Authors |
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Osama Fekry Al Balah * National Institute of Laser Enhanced Science at Cairo University. |
| Abstract |
| Background: Wound healing remains a significant clinical challenge, particularly in cases where conventional therapies prove insufficient. Recent advances in photodynamic therapy (PDT), low-level laser therapy (LLLT), and nanoparticle-based treatments offer promising alternatives for accelerating tissue repair and reducing inflammation. Objective: This study synthesizes findings from three experimental investigations examining the efficacy of methylene blue-polyethylene glycol (MB-PEG) hydrogel with PDT, chitosan nanoparticles with LLLT, and silver nanoparticles with LLLT in promoting wound healing in murine models. Methods: Data were compiled from three controlled studies involving 189 adult male CD1 and Swiss mice with standardized 6mm circular wounds. Treatment groups received various combinations of photodynamic therapy (650±5nm laser, 180mW/cm²), topical nanoparticle applications, and control treatments over 21-day observation periods. Primary outcomes included wound healing rates, histological assessment, hematological parameters, and inflammatory cytokine levels. Results: Combined therapies consistently outperformed single treatments across all studies. MB-PEG with PDT achieved 100% healing by day 21 compared to 93.28% in untreated controls. Chitosan nanoparticles with LLLT reached 100% healing versus 90% in controls. Silver nanoparticles with LLLT demonstrated 100% healing compared to 90.41% in untreated wounds. All combination therapies significantly reduced pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) at 48 hours post-treatment (P < 0.001) and showed superior histological outcomes including enhanced re-epithelialization, granulation tissue formation, and angiogenesis. Conclusions: These findings demonstrate that combination therapies utilizing LLLT with nanoparticle delivery systems or PDT significantly enhance wound healing through modulation of inflammatory responses and acceleration of tissue repair mechanisms. These approaches represent promising alternatives for clinical wound management. |
| Keywords |
| Photodynamic therapy · PEG · Methylene blue · Mice · Wound healing and Cytokines |
| Status: Accepted (Oral Presentation) |